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In 1st person past tense, write a summary of what you actually did in performing the experiment. Be sure to include any modifications. You should include enough detail so that someone could reproduce the experiment based on what you have written.
Calculations/Interpretations:
Show all math performed (label the calculation or give the formula, show your setup, and give the result) and include proper labels. Also, answer any questions asked in the lab.
1. Place the slide of onion root on the stage of the microscope.
2. Focus the microscope on the slide using the lower power objective lens. Bring the cells of the root into focus.
3. Move the slide until the section of onion root just above the root tip is in view, and search the area until cells in different phases of mitosis are in view. They will resemble the cells diagrammed in the lesson. (Telophase is often the most difficult to find.)
4. Using a high power objective, focus the microscope and search for cells in mitosis.
5. In the cell outlines that are shown draw each of the major phases of mitosis. Below each write a brief description of what your drawing shows. (Since no visible changes occur during interphase it is not included.)
6. Change the objective lens back to lower power.
7. Place a slide of whitefish blastula cells on the microscope stage and focus the microscope on the blastula.
8. Change to the high power objective and focus the microscope. Look for cells in the different phases of mitosis. Note the difference in the ends of the spindle apparatus compared to those in the onion root.
9. Below each write a brief description of what your drawing of whitefish blastula cells shows. (Since no visible changes occur during interphase it is not included.)
prophase
metaphase
anaphase
telophase
How does mitosis differ between the onion root cells and the whitefish blastula cells?
Conclusion:
Begin by stating whether the hypothesis was true or false. Use data and calculations to support your answer. Consider the following questions as you write your conclusion: Why or what happened to result in the outcome you observed? Did you learn anything new? If not, what previously concepts did this lab reinforce? Is there anything you would or could do differently that would improve the experiment? Do you have any other comments/observations you would like to share about this lab?
(*Note: Not all questions pertain to each lab.) Your conclusion should be approximately one paragraph in length.
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