What intermediate or intermediates in purine synthesis would you expect to accumulate in sulfanilamide-treated bacteria?

during the process of dna replication, brdu can replace thymidine and incorporate into the newly synthesized dna, cell proliferation thus can be reflected in the extent of brdu incorporation.

the principles and empirical processes of discovery and demonstration considered characteristic of or necessary for scientific investigation, generally involving the observation of phenomena, the formulation of a hypothesis concerning the phenomena, experimentation to test the hypothesis, and development of a conclusion that confirms, rejects, or modifies the hypothesis.