Place the following events in the correct sequence in which they would occur in a signal transduction pathway. 1. Conformational shape change occurs 2. Ligand binds with a receptor protein 3. A cascade of events amplifies and changes the signal 4. The cellular process of transcription is carried out within the cell 5. The activation, by phosphorylation, of a transcription enzyme *

both anaerobic and aerobic respiration use glucose (or other carbohydrates), proteins and lipids to produce atp and nadh. cell uses those energy molecules, obtained from food and   breaks them down during the chemical reactions of respiration. in anaerobic respiration, nadh is regenerated to nad+ by enzymes lactate dehydrogenase (lactate is produced, e.g. in muscle cells) or by alcohol dehydrogenase where ethanol is produced (alcohol fermentation), while in aerobic respiration, electrons from nadh are deposited in the mitochondria to regenerate nad+ (electron transport chain. anaerobic respiration produces 2 atp per molecule of glucose compared to aerobic respiration where about 38 atp molecules are produced.

this protocol provides a detailed procedure for total protein extraction for western blot analysis from achieved samples following trizol isolation of rna.