You prepared a series of tubes with increasing concentrations of maltose. After reading the absorbance of these tubes you prepared a Maltose Standard Curve. The equation of the line of best fit you obtained is:
y = 1.9x -0.053
You then did an experiment to study the effect of pH on amylase activity. Amylase was mixed with starch and buffer of varying pH in several tubes, which
were incubated for 10 minutes at 45°C. The DNS assay was done and absorbance readings were collected and are shown in the table:
Tube pH
Absorbance Maltose (mg/ml) Amylase Activity
(mg/ml/min)
1
0.052
2
3
3
0.339
3
5
0.899
4
7
0.393
S
9
0.018