Experiment 2.) Pidge is able to clone each GLOW gene and express these proteins in yeast. She decides to test the function of the genes in the pathway based on their protein function in vitro. Pidge has generated a primary antibody to GLOW1. Pidge wants to demonstrate that the GLOW1 protein and physically interact with the GLOW2 and GLOW4 proteins. Question 9b.) What in vitro test can she perform? (3pts)

Co-immunoprecipitation

Explanation:

Co-immunoprecipitation is a bio-technique use to analyze the physiological relevance of protein to protein interactions by binding the protein with special antibodies. With this vitro-test, a sample of a protein substance can be isolated and concentrated from thousands of other proteins.

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