What are five ways that the environment can be impacted by mining?

Across the world, mining contributes to erosion, sinkholes, deforestation, loss of biodiversity, significant use of water resources, dammed rivers and ponded waters, wastewater disposal issues, acid mine drainage and contamination of soil, ground and surface water, all of which can lead to health issues in local populations

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this protocol provides a detailed procedure for co-immunoprecipitation (co-ip) to identify protein–protein interactions.