You design primers for colony PCR with one primer binding to your gRNA insert (20 bp), and 1 primer binding to a region of your plasmid ~430 base pairs away. Your plasmid vector is pML104, which is 11,240 base pairs. In colony PCR, a positive result (indicating a colony likely carries a pML104 plasmid with the gRNA inserted) would be a PCR product that is:

Elephants in the savanna regions of africa dig holes in dried up river beds to reach water lying just below the surface. these holes provide drinking water for other animals as well. so, without the elephants, many animals might otherwise die from lack of water during the dry season. the location in which the elephants live is an example of a/n and the role they play in creating water holes is an example of a a) ecosystem; habitat b) community; niche c) habitat; niche d) niche; habitat

Food webs - transferring energy and matter from one level to another. here you see four food webs. one or more are incorrect. which food web(s) show the correct sequence of organisms, from start to top level consumer? a) a b) d c) c d) a and d

Adrought killed all the plants in an agricultural farmland. gradually, due to wind and some birds for. nearby areas, new plants started sprouting l. what stage of succession was taking place in the farmland? a) competition b) nudation c) ecesis d) reaction e) migration