An 8-mer oligopeptide has the following amino acids: A D E E F M W Y (two Es are present)
Here is your evidence:
1. Edman degradation produces A.
2. CNBr produces a 5-mer and a 3-mer that contains W.
3. Chymotrypsin produces two dipeptides and a tetrapeptide. The tetrapeptide has a total net charge of -4 at pH 12.5.
4. Staphylococcal protease in phosphate buffer produces a tetrapeptide (4-mer), dipeptide, free D, and free W.
5. Staphylococcal protease in acetate buffer produces a pentapeptide (5-mer), dipeptide, and free W.
Put in the sequence of the 8-mer (use caps here) i. e. ABCDEFGH.