Put the following 6 steps in order to indicate the overall experimental flow. a. Quantify extracted protein
b. Transform BL21(DE3) w/ pDHFR
c. Run purified protein on gel to verify by SDS-PAGE and Western Blot
d. Generate pDHFR expression vector
e. Induce DHFR protein synthesis in E. coli with IPTG
f. Purify DHRF protein by affinity chromatography (IMAC)

Question 1 of 102 pointswhich best describes adaptive radiation? oa. geographical isolation caused by an adaptationob. biodiversity resulting from few ancestorsoc. a decrease in the rate of speciationd. adaptations that organisms teach each other

Awire-hair terrier and a smooth-hair terrier are mated. the offspring produced are 6 wire-hair puppies and 2 smooth-hair puppies. a. what pattern of inheritance best describes this situation? b. what is the genotype(s) of the wire-haired puppies? of the smooth-haired puppies? c. what would be the expected genotype and phenotype ratios of a mating between 2 wire-haired terriers that are heterozygous?

How and why organisms are hierarchically classifies