Which type of mutation would have a worse affect on the protein, a substitution of an insertion?

A missense mutation is when the change of a single base pair causes the substitution of a different amino acid in the resulting protein. This amino acid substitution may have no effect, or it may render the protein nonfunctional.

a scenario where a cell may be needed to perform a form of endocytosis is when transporting large molecules. a scenario where a cell may be needed to perform a form of exocytosis is when releasing the large molecule from the cell.