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You wish to express a particular peptide in E. coli using a high copy number plasmid. You have the amino acid sequence for the peptide. a. Explain the experimental process for generating and selecting the genetically engineered E coli using restriction enzymes, ligase, E. coli, plasmid with neomycin resistance, and known amino acid sequence. b. What control elements would you place on the plasmid to regulate expression and to prevent read-through
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You wish to express a particular peptide in E. coli using a high copy number plasmid. You have the a...
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