What two organ systems control the negative feedback systems of the body?

The nervous system and the endocrine system controls the negative feedback system of the body.

The answer to this will be from your lab notes/procedure. it would be best to ask another student who participated in the lab as we do not know what was done, what enzyme or if the temperature is celsius or fahrenheit. if i were to guess though i'd say the rate stopped due to enzyme denturing at high temperature

Iwould say overuse ( but not 100% sure ) in the u.s. we tend to overuse water by leaving it running, throwing it away, taking long showers, etc.