10 steps of protein synthesis in order

1.) DNA unzips in the nucleus in interphase.

2.) mRNA nucleotides transcribe the complementary DNA message.

3.) mRNA leaves nucleus and goes to ribosome.

4.) mRNA attaches to ribosome and first codon is read

5.) tRNA with anticodon UAC brings in proper amino acid from cytoplasm.

6.) a second tRNA brings in new amino acids.

7.) Ribosome adds amino acid to the first acid with a peptide bond.

8.) Ribosome slides to next codon for translation.

9.) tRNA released into cytoplasm until needed again .

10.) tRNA returns to ribosome adds amino acid to growing polypeptide chain.

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this protocol provides a detailed procedure for agarose-based ip to purify and enrich the protein of interest.