Biology, 25.02.2021 19:20 morrisjillian23

A student prepares a cell homogenate from spinach leaves. She performs a cell fractionation experiment to determine the shortest amount time of centrifugation at 2000xg that will yield the highest percentage of chloroplasts in the pellet fraction. She splits the spinach homogenate into 5 equal parts and centrifuges the parts at 2000xg for 0, 5, 10, 15 or 20 min. She then determines the proportion of chloroplasts in the supernatant and pellet fractions. The controlled variable(s) in the student's experiment is/are:. a. proportion of chloroplasts in each fraction.
b. use of same plant homogenate throughout.
c. time of centrifugation in minutes.
d. g-force of centrifugation.

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A student prepares a cell homogenate from spinach leaves. She performs a cell fractionation experime...

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