You are performing site directed mutogenesis to test predictions about which residues are essential for protein function, which of each pair of Amino acid substitute listed below will you expect to disrupt protein structure the most. Explain 1) isoleucine replaced by alanine or phenylalanine
2) lycine replaced by glutamine or arginine
3) asparagine replaced by glutamate or glutamine
4) proline replaced by histidine or glycine