The 3-dimensional structure of RNA polymerase, the enzyme that catalyzes formation of the phosphodiester bond during RNA synthesis (i. e. transcription), was determined using X-ray crystallography. What is the correct chronological order of steps
describing how this was possible using this technique? 3 pts
#1: Aim an X-ray beam through the crystal, atoms that comprise your protein that is crystallized diffract the X-rays.
#2: Determine the pattern of x-rays (X-ray diffraction pattern) using a digital detector.
#3: Using data from the x-ray diffraction pattern and amino acid sequence, build a 3-D model of the protein using computer
software.
#4: Obtain a pure sample of protein that is very concentrated and therefore forms a crystal.
A/ #1 ->#2 -> #3 -> #4
B/ #3 -> #4 -> # 1 -> #2
C/ #4 ->#3 ->#1 -> #2
D/ #4 -> #1 -> #2 -> #3