Dr. Serena Velazquez lands a coveted post-doc position at Johns Hopkins following completion of her MD/PhD in cancer therapeutics to treat late-stage pancreatic cancer using a mouse model. Her post-doc will be devoted to targeted mutagenesis of replicative enzymes to halt cell division in cancerous cells. She reviews mitosis and meiosis before reporting for her first day of work. 1. Dr. Velazquez considers targeted mutagenesis of DNA ligase. What would that do?
A. Prevent dsDNA from forming ssDNA.
B. Prevent the leading strand from being able to unite together Okazaki fragments.
C. Prevent the lagging strand from being able to unite together Okazaki fragments.
D. Prevent replication of DNA.
2. If Dr. Velaquez wants to target the replication fork of synthesizing DNA in eukaryotes, what proteins might she consider knocking down to see the effect on cancer progression?
A. SSB.
B. LOL.
C. LOF.
D. RPA.