A model of gene expression within the lac operon is shown here. If a mutation occurs in the operator (O) region of the operon that changes the sequence of nucleotides in the operator, which statement(s) predict the outcome of this mutation? Select ALL that apply.

An enzymatic hydrolysis of fructose-1-p, fructose-1-p(aq) + h2o(l) - fructose (aq) + pi (aq) was allowed to proceed to equilibrium at 25°c. the original concentration of fructose-1-p was 0.2 m, but when the system had reached equilibrium, the concentration of fructose-1-p was only 6.52 x 10^-5 m. calculate the equilibrium constant for this reaction and the free energy of hydrolysis of fructose-1-p.