Biology, 06.04.2021 01:00 jadejordan8888
In the ELISA procedure, first a protein sample is immobilized to a plastic well. Then, the antibody to the protein of interest is added, incubated, and the excess is washed out. Next, the detection antibody (which binds to the primary antibody) is added, incubated, and the excess is washed out. The last step is to add the detection dye (which binds to the detection antibody) to the wells and allow it to incubate. What do you predict the result would look like if you added the detection antibody first, then the antibody to the protein of interest
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