1. You have set up a cell-free transcription system in a test tube. By mixing DNA, RNA polymerase, sigma factors, ribonucleotides and other essential substances you are able to make mRNAs identical to those made under a variety of conditions in the cells from which these components were isolated. What would happen if you were able to remove the sigma factor first added to the tube and replace it with another sigma factor

The dna in a cell’s nucleus encodes proteins that are eventually targeted to every membrane and compartment in the cell, as well as proteins that are targeted for secretion from the cell. for example, consider these two proteins: phosphofructokinase (pfk) is an enzyme that functions in the cytoplasm during glycolysis. insulin, a protein that regulates blood sugar levels, is secreted from specialized pancreatic cells. assume that you can track the cellular locations of these two proteins from the time that translation is complete until the proteins reach their final destinations.for each protein, identify its targeting pathway: the sequence of cellular locations in which the protein is found from when translation is complete until it reaches its final (functional) destination. (note that if an organelle is listed in a pathway, the location implied is inside the organelle, not in the membrane that surrounds the organelle.)

Environmental impacts on evolution : mastery test

Amino acids contain the elements carbon, hydrogen, oxygen, nitrogen, and sometimes sulfur. of the 20 amino acids found in humans, 11 are called "nonessential" because they can be manufactured by the body when needed. which elements in these 11 amino acids are commonly obtained from the metabolism of sugar molecules?