A physical disruption to an ecosystem occurred when a highway was built through a forest. Which of the following would provide evidence that the addition of a highway to this forest affected populations? The amount of water runoff from higher elevations has increased after the highway was built.

The trees that were not cut down during the construction of the highway have continued to grow.

There are fewer species of nesting birds living in the forest after the highway was built.

A greater area of the forest floor is now exposed to the sunlight after the highway was built.