Effects of increasing concentration of enzyme (lysozyme) on initial velocity (vo). Construct vo vs [E] (mg) plot. 2. The effects of increasing concentration of substrate (bacterial cell wall) on initial velocity (vo). a) Construct vo vs [S] plot and estimate the Km and Vmax. b) Construct Lineweaver-Burk (1/vo vs 1/[S]) and determine the Km and Vmax 3. Explain briefly the significance of Km and Vmax. 4. Effects of increasing temperature on initial velocity. Construct vo vs Temperature (OC) plot.

The return of the wolf to yellowstone national park will probably affect the bird population to some degree, not significantly. while wolves will hunt birds when necessary, they prefer to hunt larger prey. the bird population will most likely only be affected when hunting is scarce for the wolves. birds of prey will also probably not be affected as much as other birds who spend more time on the ground hunting for food as the tend to spend more time in the air or in trees since they do not need to stay on the ground for long due to the nature of the way they hunt.