At what percentage will the allele frequency of a particular trait represent the least amount of genetic variation?

It seems that you have missed the necessary options for us to answer the given question above, but anyway, here is the answer. The percentage that allele frequency of a particular trait will represent the least amount of genetic variation is 100% as there would only be that allele in the population there would be no variation. Hope this helps.

If it doesn't have a nucleus its a prokaryote