Why are two different restriction enzymes used to cut the puc19 plasmid and the lux gene dna? what would have happened if only hind iii enzyme was used

How do high-energy electrons from glycolysis and the krebs cycle contribute to the formation of atp from adp in the electron transport chain? a. high-energy electrons interact with pyruvic acid to create a phosphate bond with adp, forming atp. b. high-energy electrons supply the needed energy to synthesize atp from the lower-energy adp in the electron transport chain. c. high-energy electrons supply a negative charge which causes the citric acid to oxidize, forming a phosphate group to bond with adp. d. high-energy electrons increase the temperature of adp so it easily reacts to form atp.