Which experimental design decision would most likely produce invalid and unreliable results

The tasmanian devil, a marsupial carnivore, is facing extinction due to devil facial tumor disease (dftd) which causes bulging cancerous lumps and lesions to erupt around the face and neck — often causing enough deformation to make seeing or eating difficult. dftd has evolved into a contagious cancer, a trait that is unique among cancers. devil mating behavior involves biting around the head and neck, allowing cells from one individual — especially cells from the crumbly dftd tumors — to be transferred to the wounds or face of a new individual. this marsupial was once found across australia, but sea levels rose, isolating the tasmanian population, while the australian population went extinct. what would be an outcome of genetic isolation that is likely to have impacted the spread of dftd? a) reduced territory puts diseased individuals in greater contact with non-diseased ones. b) inbreeding results in less variation in facial features so the cancer is generally fatal. c) genetic isolation has made it difficult for scientists to develop a vaccine against dftd. d) the lack of genetic variation in the immune system of tasmanian devils minimizes resistance to the disease.

Legumes, a type of plant, require rhizobia, a type of soil bacteria, to survive since these organisms fix nitrogen during photosynthesis. rhizobia use the legumes for food. what would most likely happen to the legume population if rhizobia suddenly became extinct?

Which represents the cross between parent plants if one is heterozygous for yellow-colored pods and the other is homozygous for green-colored pods? yy  ´    ´    ´    ´  yy