Growth of human hepatoma cell lines with differentiated functions in chemically defined medium

A human hepatoma cell line, HuH-7, which was established from a hepatocellular carcinoma, was found to replicate continuously in a chemically defined medium when the medium was supplemented with Na2SeO3. The cells grew better in this medium than in serum-containing medium without any adaptation period. Other established human hepatoma and hepatoblastoma cell lines, HuH-6 cl-5, PLC/PRF/5, huH-1, and huH-4, also grew in the defined medium. Although HLEC-1 cells failed to proliferate continuously with Na2SeO3 alone, they grew if a cell-free conditioned medium from HuH-7 cells was added to the medium. These cell lines, except the HLEC-1 cell line, produced the following human plasma proteins among those examined: albumin, prealbumin, α1-antitrypsin, ceruloplasmin, fibrinogen, fibronectin, haptoglobin, hemopexin, β-lipoprotein, α2-macroglobulin, β2-microglobulin, transferrin, Complement Components 3 and 4, and α1-fetoprotein. Beside plasma proteins, the media from HuH-7, HuH-6 cl-5, PLC/PRF/5, and huH-1 contained anti-carcinoembryonic antigen-reactive proteins, and those from PLC/PRF/5, huH-1, and huH-4 medium contained hepatitis B surface antigen. These proteins were detected during periods of serial cultivation over 9 months under the above culture conditions. The hepatoma cell lines grown in the fully defined synthetic medium may provide a new approach for investigating the growth and metabolism of human hepatoma cells in vitro.

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The return of the wolf to yellowstone national park will probably affect the bird population to some degree, not significantly. while wolves will hunt birds when necessary, they prefer to hunt larger prey. the bird population will most likely only be affected when hunting is scarce for the wolves. birds of prey will also probably not be affected as much as other birds who spend more time on the ground hunting for food as the tend to spend more time in the air or in trees since they do not need to stay on the ground for long due to the nature of the way they hunt.