subject
Biology, 01.05.2021 01:30 herchellann302

The genome of a newly discovered bacteriophage is a linear DNA molecule 10,500 nucleotide pairs in length. One sample of this DNA has been incubated with restriction enzyme X and another sample with restriction enzyme Y. The lengths (in thousands of base pairs) of the restriction fragments produced by the two enzymes have been determined by gel electrophoresis to be as follows: Enzyme X: Fragment X1 = 4.5; X2 = 3.6; X3 = 2.4
Enzyme Y: Fragment Y1 = 5.2; Y2 = 3.8; Y3 = 1.5

Next, the fragments from the enzyme X reaction are isolated and treated with enzyme Y, and the fragments from the enzyme Y reaction are treated with enzyme X. The results are as follows:

X fragments treated with Y: X β€” 1 β†’ 4.5 (unchanged)
X βˆ’ 2β†’2.1 + 1.5
X βˆ’ 3β†’1.7 + 0.7
Y fragments treated with X:Y βˆ’ 1β†’4.5 + 0.7
Y – 2β†’2.1 + 1.7
Y βˆ’ 3β†’1.5 (unchanged)

Required:
Draw a restriction map of the phage DNA, indicating the positions of all enzyme X and enzyme Y restriction sites and the lengths of DNA between them.

ansver
Answers: 2

Another question on Biology

question
Biology, 21.06.2019 18:30
How is the male reproductive system different from other body systems? it isn’t necessary to vital signs. it monitors other body systems, and adjusts if necessary. it transports items from one system to another. it is the only system that produces hormones in the male.
Answers: 3
question
Biology, 22.06.2019 05:00
Cedric has a low fever and minor aches. yesterday, he went to the doctor for his booster shot and receive the flu shot. which best explains why he has this reaction?
Answers: 2
question
Biology, 22.06.2019 06:00
Which example would the nurse consider to be a part of systems thinking theory?
Answers: 1
question
Biology, 22.06.2019 08:30
What was darwins theory of common decent
Answers: 3
You know the right answer?
The genome of a newly discovered bacteriophage is a linear DNA molecule 10,500 nucleotide pairs in l...
Questions
question
Mathematics, 06.12.2020 20:20
question
History, 06.12.2020 20:20
question
Geography, 06.12.2020 20:20
question
History, 06.12.2020 20:20
Questions on the website: 13722361