Use this information to match the list of probes to the 5 samples below: 4-8. You have isolated several DNA sequences from a variety of mouse tissues. You have labeled each one of them with a radioisotope and will use them as probes on blots of several DNA and RNA samples.
Below are a list of all the probes you generated (probes A through F) and a list of all the DNA and RNA samples that you will analyze (Samples 1 through 5).
Beside each sample, write the letters corresponding to all the probes that will bind to a complementary sequence in that sample. If you think that no probe will bind to a complementary sequence in a certain sample, write an "X" beside it.
List of probes
Probe A: promoter sequence of a gene that is only expressed in the nervous system
Probe B: promoter sequence of one of the genes encoding a ubiquitously expressed histone protein
Probe C: coding sequence of a gene that is only expressed in the nervous system
Probe D: coding sequence of one of the genes encoding a ubiquitously expressed histone protein
Probe E: intron of a gene that is only expressed in the nervous system List of samples
Sample 1: Southern blot of DNA extracted from adult mouse epidermal cells
Sample 2: Southern blot of DNA extracted from adult mouse cardiac muscle cells
Sample 3: Northern blot of RNA extracted from adult mouse epidermal cells
Sample 4: Northern blot of RNA extracted from adult mouse nervous system cells
Sample 5: Northern blot RNA extracted from adult mouse cardiac muscle cells