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Draw a restriction map of the multiple cloning site of pUC18 that shows how luxAB is expected to ligate into the vector (the map only needs to show the restriction sites used for this set of experiments). For bioluminescence activity to be observed, the genes must get transcribed before they gets translated. Transcription is typically initiated by a transcriptional promoter. What is the difference between what serves as the promotor for lux operon expression in the lux colonies and what serves as the promoter for luxAB expression in luxAB colonies
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Draw a restriction map of the multiple cloning site of pUC18 that shows how luxAB is expected to lig...
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