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Using and spectrophotometer, you measure 2 dilutions of NADH, and get absorbance values of 0.4 for sample A, and 0.2 for sample B. You know that the path length is 1 cm, and the extinction coefficient for NADH is 6220 (L Morcm). Using the Lambert-Beer Law equation (below), calculate the concentrations of sample A Select] and Sample B (Select ] A = log10 () = Ecl Where: A- Absorbance C- Concentration (mol 1 - Path length (cm) E = molar decadic extinction coefficient L mol. cm 1o - Intensity of the incident light 1 - Intensity of the transmitted Night
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Using and spectrophotometer, you measure 2 dilutions of NADH, and get absorbance values of 0.4 for s...
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