While testing amylase activity, John uses a buffer of pH 6 and substrate starch. He incubates them at 104ºF (40ºC). All the tubes show glucose formation, except one, which had no starch solution. Why did this happen?

What would most likely happen if a person increased the amount of saturated fat in his or her diet? the person's risk of cardiovascular disease would increase. the person's risk of cardiovascular disease would decrease. the person's bad cholesterol would decrease. the person’s good cholesterol would increase.