Biology, 17.07.2021 01:50 samchix727

An ampicillin-resistant, tetracycline-resistant plasmid, pBR322, is cleaved with PstI, which cleaves within the ampicillin resistance gene. The cut plasmid is ligated with PstI digested Drosophila DNA to prepare a genomic library, and the mixture is used to transform E. coli K12. Which Antibiotic should be added to the medium to select cells that have incorporated a plasmid

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Answer from: NidaFurqan

tetracycline

Explanation:

A plasmid is a small, circular, double-stranded DNA molecule, which is physically separated from chromosomal DNA and is able to replicate independently. Moreover, plasmid transformation is the process by which exogenous DNA is introduced into a cell (usually a bacterial host cell). In this case, Escherichia coli K12 cells were transformed with the pBR322 plasmids that encode both ampicillin and tetracycline resistance genes. According to data, plasmids were firstly cleaved with the PstI type II restriction endonuclease within the ampicillin resistance gene, thereby these plasmids lose the ampicillin-resistance gene that allows selecting E. coli K12 cells by their resistance to ampicillin. In consequence, tetracycline is the only one selective marker that will indicate that the bacteria have incorporated the plasmid.

Answer from: Quest

answer : haversian

[explanation: -(compact bone is organized as parallel columns, known as haversian systems, which run lengthwise down the axis of long bones. these columns are composed of lamellae, concentric rings of bone, surrounding a central channel, or haversian canal, that contains the nerves, blood vessels, and lymphatic system of the bone)]

Answer from: Quest

Atp is is our little "pocket" of energy. of course, if you have too little, then it means you have too little energy, and you'll pass out. the way to not have enough, is to have no energy sources (glucose, fats, proteins). you can't have too many atp molecules. cycles like the krebs cycle produce it, but if you have too much of any substrate (or too little for that matter), the cycle stops and atp is no longer made (it stops until substrate levels are such that the process can go on). in any case, this is unlikely since atp is constantly hydrolyzed and remade, even while you're asleep. you won't have too much. if you have zero, you are dead or dying.

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An ampicillin-resistant, tetracycline-resistant plasmid, pBR322, is cleaved with PstI, which cleaves...

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