15. Assuming the fluorescence threshold is set to be 1. Which gene has the highest gene expression rate? What is the Ct value? Gene with highest expression = Ct value =

The correct answer is - Gene 1 and 14.5 (or 14).

Explanation:

The cycle threshold is denoted as Ct value. It is the cross point at the threshold line meets with fluorescence signal reaction curve. In the given RTpcr experiment, the threshold line is fluorescence intensity 1 thus, Ct value for each gene will be the point at which each curve meets the fluorescence intensity line at 1. Ct value of is inversely proportional to amount of nucleic acid in the given sample.

Here, the Ct for gene 1 is the lowest, so the amount of DNA would be highest.

Gene with highest expression= Gene 1

Ct value= 14.5

Proteins are the workhorses of the cell, controlling virtually every reaction within as well as providing structure and serving as signals to other cells. proteins are long chains of  amino acids  , and the exact sequence of the amino acids determines the final structure and function of the protein. instructions for that sequence are encoded in  genes  . to make a particular protein, a messenger ribonucleic acid (mrna) copy is made from the gene (in the process called  transcription  ), and the mrna is transported to the  ribosome  . protein synthesis, also called  translation  , begins when the two ribosomal subunits link onto the mrna. this step, called initiation, is followed by elongation, in which successive amino acids are added to the growing chain, brought in by transfer rnas (trnas). in this step, the ribosome reads the  nucleotides  of mrna three by three, in units called  codons  , and matches each to three nucleotides on the trna, called the anticodon. finally, during termination, the ribosome unbinds from the mrna, and the  amino acid  chain goes on to be processed and folded to make the final, functional protein