You wish to develop a monoclonal antibody (mAb) that binds with high affinity to the SARS-CoV-2 spike (S) protein, for use as a therapeutic agent. You have isolated a human B-cell clone that produces an IgG that is specific for the SARS-CoV-2 S protein, but its affinity for binding the target epitope is low. Describe in conceptual detail how you could use a protein engineering approach to enhance the affinity of the mAb for the target antigen.
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