Using power point (or equivalent) or by drawing, make a crude cartoon showing the results from a SAR1 western blot from cells treated or not with GTPgammaS, then lysed either with SDS detergent or by sonication without detergent (to preserve ER integrity) then centrifgued briefly to pellet cell membranes cell treatment (before lysis) Fraction run on SDS gel Method of lysis
None (control) Pellet SDS detergent
None (control) Supernatant SDS detergent
GTPgammaS Pellet SDS detergent
GTPgammaS Supernatant SDS detergent
GTPgammaS Pellet Sonication (no detergent)
GTPgammaS Supernatant Sonication (no detergent