The researcher places the lacZ gene in the cloning region so that it is disrupted by DNA inserts. Place the remaining steps for this process in the correct order. Not all steps will be used. a. Blue colonies that survive On the culture medium contain the recombinant plasmid.
b. Grow cells containing the recombinant plasmid in a medium with ampicillin and X-gal (a galactose derivative).
c. All the fragments have the sticky ends 5 '—GCGGCCGC—3'.
d. All the fragments have identical sticky ends: 5' —GGCC—3'.
e. Use DNA ligase to join the sticky ends of the DNA sample and the plasmid.
f. White colonies that survive on the culture medium contain the recombinant plasmid.
g. Remove colonies containing the recombinant plasmid from the culture medium and continue growing.

cellular respiration oxidizes food into energy in the form of atps.

glycolysis (6 c hexose) > 2x atps are used > 6c hexose biphosphate > 2 x 3c triphosphate >

2 x 3c pyruvate (2x nadh produced from nad) and (4x atp produced)

pyruvate actively pumped mitochondrial matrix.

picture a world where computers are building made-to-order personalized robotic arms and synthetic organs, drones are circulating above mars! due to the rapid growth of technology, the future has never been more unpredictable, and it presents a huge challenge in education: we are teaching students how to work in a future that we have never been unsure of. it is widely predicted that in eight years’ time, there will be more jobs in the fields of science, mathematics, technology and engineering than in any other discipline. and, as the technology continues to develop, it will soon become clear that addressing social problems will involve more innovative thinking, and analytical and technical knowledge than ever before. undeniably, there are no subjects that encourage innovative and creative thinking, deep thought, problem solving and independence more than the stem subjects. while it is widely commented on that there is a need to reimagine the ways stem subjects are taught in schools by embracing a more interactive and innovative approach to appeal to more students, it should come as no surprise that some of the most reputable educational organizations in the world are already implementing changes to embrace stem. harvard, for example, announced in 2014 that they were increasing their computer science faculty by over 50% and has since introduced computer science as a core subject for all freshmen. the urgency of supporting stem subjects at a young age is becoming more relevant than ever, as adopting a keen interest in the curricula needs to happen early. however, beyond the age issue is one of gender.

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it marks things for degradation via the proteasome. it alters there celluar location.