Biology, 13.08.2021 02:10 abby894927
Several mutational changes to proteins involved in apoptosis are listed below. For each, indicate whether you expect it to have a dominant-negative phenotype. In other words, do you expect it to cause a defect in apoptosis even in cells that also contain the normal protein?
A. Deletion of death domain of Fas death receptor.
B. Mutational change to Fas ligand that prevents its oligomerization.
C. Deletion of the death effector domain of FADD.
D. Deletion of the death effector domain of procaspase-8.
E. Deletion of the CARD domain of Apaf-1.
F. Mutation of the catalytic cysteine of caspase-8 to serine.
G. Mutational change to Bax that prevents its oligomerization with itself and with Bak.
H. Mutational change to IAP that increases its affinity for caspases.
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