Lab CUA
calls
that
are used
that
live long to
hormone
drug giver
to promote
/
more
WBC
Experimental Design and Data Analysis
1. A fearcher would like to stndy the effects of Granulocyte Colony Stimulating Factorte-est) on 32D cells
, a
murine hematopoietic cell line. What does this statement mean?
to grow is
hered
more
formation of blood calls
2. In the laboratory, the researcher begins by using varying concentrations of G-CSF to treat 32D cells for various
periods of time. Why would the researcher have to do this?
3. Below are some results. Explain why the researcher repeated the experiment 3 times.
mo
mD
100
WT
100
100
10-
10
Fold Increase
Fold Increase
Fold increase
6
Time (days)
Time (days)
Time (days)
Cell proliferation/survival analysis of 32D clones using 100 (). 10 (), 1 ( * ), and 0.1 () ng/ml G-CSF.
WT (wild type), mD and mO are G-CSF-R mutants.
4. What can you conclude from these data?
5. Compare and contrast the data from this experiment below.
1000
100
Cell Count (x 10$/mL)
10
1
0.1
0 1 2 3
4 (days)
Parental 32Dc13 cells (solid symbols) and 32D/ɛ cells (open symbols) maintained in IL-3 were washed with PBS
and transferred to medium containing 25 U/mL IL-3 (circle), 50 ng/mL G-CSF (square), or no cytokines (triangle).
Viable cells were counted using the trypan blue dye exclusion method.