Which of the following is needed in order to direct CRISPR-Cas9 to cut the specific area of the genome you are trying to manipulate? a. a specific palindromic sequence in the targeted area of the genome
b. multiple restriction sites in the targeted area of the genome
c. an sgRNA molecule designed to be complementary to the targeted area of the genome
d. DNA ligase
e. ALL OF THESE

He did not infringe upon the policy making powers that he felt the constitution gave congress. but the determination of foreign policy became preponderantly a presidential concern. when the french revolution led to a major war between france and england, washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-british. rather, he insisted upon a neutral course until the united states could grow stronger. which provides the best objective summary of this excerpt? president washington made a good choice when he decided not to accept the recommendations of his advisors and instead insisted that the united states stay neutral during the war between france and england as president, washington believed that the united states should remain neutral in foreign policy even though he had advisors who recommended that he take sides during the war between france and england. washington did not make policy decisions during his presidency because he did not want to interfere with the policy-making powers that the constitution gave congress. washington refused to accept entirely the recommendations of either his secretary of state thomas jefferson, who was pro-french, or his secretary of the treasury alexander hamilton, who was pro-britis

Drag each tile to the correct box. the body monitors the levels of oxygen in the blood to regulate breathing. isabel is running in a marathon and is near the finish line. she feels out of breath. how will her nervous system work to generate a reaction? arrange the tiles in chronological order. isabel's breathing rate increases. sensory receptors in the arteries detect low oxygen levels. the brain sends signals through motor neurons. sensory neurons generate an impulse. the central nervous system relays an impulse to certain brain regions.

Differentiate renewable and nonrenewable resources