We will be using several restriction enzymes to analyze our PCR products using a restriction fragment length polymorphism analysis. Locate the positions were EcoRI, EcoRV, Nsil, and BamHI cut the plasmid. Will these enzymes cut on both sides of the insert to cut it back out? what will happen if we use an enzyme that cuts at only one site?

they each offer a fresh glut of resources to the organisms that can arrive and exploit them. as individuals and new species continue to appear, they actively and passively alter the environment. competition for shared resources increases, local abiotic and biotic conditions shift, and the relative abundances of species change accordingly. the process is called ecological succession, and it is one of the earliest and most well-known concepts in ecology.

ans.

dna fingerprinting is defined as a process that determines the dna characteristics of an individual. these dna characteristics are unique among individuals as their fingerprints, so it is known as dna fingerprinting.

steps of dna fingerprinting includes extraction of dna from sample, amplification of dna, restriction digestion of amplified dna, and then analysis of dna by gel electrophoresis, respectively.

thus, the next step performed by the scientist is 'to cut the amplified dna (obtained by polymerase chain reaction) by restriction enzymes that cleave the dna into fragments and their analysis.'

answer: andy garner, a 17-year-old who decided to become a human guinea pig in 1965 and go without sleep for 11 days. by the second day his eyes had already stopped focusing, and he lost the ability to identify objects by touch. by day three he was moody and uncoordinated and by the end he had trouble with short term memory and had even started hallucinating.

and a rare genetic mutation called fatal familial insomnia, where people can’t sleep at all, gets progressively worse over months or years and leads to dementia and death.

a devoted soccer fan died after staying awake 48 hours to watch the world cup, she said. ‘while his untimely death was due to a stroke, studies show chronically sleeping fewer than six hours a night increases stroke risk by 4 1/2 times compared with those getting a consistent seven to eight hours.'

*shorten it up as your wish*

(i have divided it into 3 simple paragraphs)

the correct answers are;

a. the rock was made from more than one type of material.

e. layers of hot liquids in the rock cooled and solidified at different temperatures.

these are the two options that are related to the statement given above.