You are planning to split a T25 flask of CHO cells that contained 5 ml of media. You removed all of the old media and washed the cells with PBS. The PBS was then removed and 1 ml of trypsin was added to disloge the cells. After 10 minutes, 4 ml of fresh media was added to the flask. 100 ul of cells were removed from the flask and added to 700 ul of fresh media in a microfuge tube. The tube was mixed and then 100 ul of cell suspension was removed and added to 100 ul of trypan blue. Using the countess you received a count of 4.5 x 10^4 cells/ml. What is actual concentration of cells in the T25 flask, after the trypsin and fresh media was added