Before the development of electrophoresis to separate macromolecules, was used. Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a organic extraction methods b column purification c high speed centrifugation d PCR

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step 1: nitrogen fixation- conversion of atmospheric no₂ into forms useful to plants by lightning, bacteria, and cyanobacteria.

step 2: nitrification-process of converting ammonia to nitrite or nitrate.

step 3: assimilation-plant roots absorb new form of nitrogen.

step 4: ammonification-conversion of organic nitrogen to ammonia by bacteria involved in decomposition.

step 5: denitrification-bacteria turns the decomposed matter back into n₂o, which is released into atmosphere; repeat cycle.