What tool or resource is useful for identifying previously unknown protein-coding genes in a genomic DNA sequence

Over the past 60 years, many amphibian species have experienced significant population declines and some species have become extinct. scientists suspected that local human activities such as the destruction of wetlands, regional pollution, and deforestation were the main reasons for these losses. however, research over the past 20 years reveals significant amphibian population declines in protected areas of the world, such as nature preserves and parks. these global declines suggest widespread problems including increased ultraviolet radiation, acid rain, and disease. in switzerland, for example, 14 of the 20 native amphibian species are threatened with extinction. chytridiomycosis is a fungal disease first identified in 1998 as a cause of massive amphibian deaths. in some severely impacted populations, a few individuals have survived, perhaps because of some natural resistance. if these resistant individuals continue to survive and prosper, new resistant populations might emerge. this would be an example of the founder effect artificial selection genetic drift natural selection sexual selection

How does this experiment explain why it is often milder in areas such as coastal maryland while areas such as kansas or iowa have more extremes in temperature

Below, the levels of organization in a multicellular organism are shown from least to most complex. which level of organization can be described as several different types of tissues working together to perform a common task?