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Biology, 02.02.2022 20:10 jaylinthornton6

A small peptide was found to contain equimolar amounts of the following amino acids: arginine, glutamic acid, glycine, lysine, methionine and phenylalanine. Individual samples of the peptide were treated with the following agents with the results noted: Reagent Specificity Trypsin Lys, Arg (C) Chymotrypsin Phe, Trp, Tyr (C) Staphylococcus V8 protease Glu, Asp (C) Pepsin Phe, Trp, Tyr (N) Cyanogen bromide Met (C) a) trypsin: arginine and a pentapeptide b) cyanogen bromide: two tripeptides c) S. aureus V8 protease: lysine and a pentapeptide d) chymotrypsin: a dipeptide and a tetrapeptide, with the latter showing absorbance at 280nm. What is the primary structure of the peptide? Explain each piece of evidence given and the reasoning that led to your answer.

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