Calculate the transformation efficiency given an initial concentration of 0.006ug/ul plasmid and the following:
200ul CaCIa
250 ul LB broth
15 ul of plasmid added to culture
150 ul of solution from the reaction tube spread on agar plate
50 colonies on the + plasmid ampicillin plate

if you listed your answers right then i believe your answer is b.

the answer to the blank provided is “an autosomal gene that is required for the expression of genes on the x chromosome”.

testis-determining factor, abbreviated as td, which is more popularly known as sex-determining region y or sry protein, is a dna-binding protein or  gene-regulatory protein/transcription factor, encoded by the  sry gene. it is the gene responsible for the initiation of male gender determination in humans.