After the secondary stain has been added, gram-positive organisms are stained and gram-negative organisms are stained

(Assuming that this stain was done with crystal violet and safarin) 

Gram-positive will appear blue to dark purple. 
Gram-negative will appear orange, red, or pink. 

Cell division is important to single celled organisms because it allows the cells to reproduce. through cell division, cells make exact copies of themselves. when single celled organisms go through cell division, they make exact copies of themselves which is how they reproduce.

The casefor proofs