What are some of the limitations in microscope technology?

Microscope technology gives us access to some pretty important/powerful information, however some limitations to it include: resolution limit, low magnification, & poor surface view,

 the wavelength of the light limited the resolution of traditional microscopy to around 0.2 micrometers. In order to gain higher resolution, the use of an electron beam with a far smaller wavelength is used in electron microscopes.

In my own word cell technology can  lead to advances in the understanding of diseases mechanism and transmission because diseases is very bad