List two differences you would expect to see in the composition of lipids in the e. coli membrane when the cells are incubated at 25 ∘c compared to incubation at 37 ∘c.

At 37 degree centigrade, phosphorus quantity is decreased while there is accumulation of phosphorus at 27 degree centigrade in cell membrane of E.coli. There is a change in glycerol to phosphate molar ratios which indicates that phosphatidyl glycerol phosphate replaced phosphatidyl glycerol during the late growth stages of E.coli when grown at 27degree centigrade.

C14 dating since carbon is present in both living and no living objects .