To clone the entire coding region of p53 into the plasmid, which restriction enzyme(s) could you use to cut both your pcr product and the pcdna3 plasmid?

They protect bacterial cells from invasion by foreign DNA. Plasmid vector you want to use to clone a gene. The diagram shows the location of the recognition sites for four restrictions enzymes, BamHI. As the bacteria divide they will lose the plasmid. If a plasmid lacks a roots of replication.

BamH1 and EcoR1

To clone the p53 segment into the pcdna 3 plasmid we can use BamH1 and EcoR1 restriction enzymes. The plasmid contains the restriction sites for BamH1 and EcoR1 to insert the desired segment which ever you want to clone. You have to use the same restriction enzyme to cut both the pcr product(gene) and the plasmid, so that the both will have susceptible sites for one another.

We can use any other enzymes too but that should have restriction site in between the plasmid inserting regions. But have to use the same for the pcr product which we have to clone in the plasmid.

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