Smoking  ,sitting  too  long  drinking  too  much alcohol, and eating too many foods high in saturated and trans fats are examples of that offset the wellness benefits of fitness programs.

Smoking, sitting too long, are examples of RISK FACTORS that offset the wellness benefits of fitness program.
Fitness program has many benefits that improve one's health status, but these benefits may be hindered from manifesting if one is indulging in some habits or eating some kind of foods that are not beneficial to one's health.  Such unhealthy habits and foods are termed risk factors. And these risks factors may increase the probability of one having some  types of diseases in the future. 

Pro insulin undergoes extensive posttranslational modification along the production pathway .